ShrimProtect IMNV
ShrimProtect RxReady IMNV qRT-PCR Kit utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect Infectious Myonecrosis Virus (IMNV) in various samples in under 45 minutes.
Analytical Performance
Limit of Detection (LoD): 0.25 copies/µL reaction.
Good linearity (R2 > 0.99) from 100,000 copies/reaction until 10 copies/reaction.
Good linearity (R2 > 0.99) from 100,000 copies/reaction until 10 copies/reaction.
Sample Type
Purified RNA from broodstock, shrimp fries, shrimps, artemia, polychaeta, water, mud, and feed.
Instrument Compatibility
QuantStudio 5
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red-X, and Cy5 channels
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red-X, and Cy5 channels
Features
- ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
- Open System: Use any existing qPCR system that you have
- Speedy: Only 43 minutes PCR time (with QuantStudio 5).
Product Details
ShrimProtect RxReady IMNV qRT-PCR Kit has been validated by Balai Pengujian Ikan & Lingkungan (BPKIL) and has acquired certificates NIE KKP RI D 2305720 BBC to specifically detect target gene ORF1 of the IMNV. The kit also contains Internal Control for shrimp.
Procedures
Store the reagents at -20oC upon delivery.
When ready to use, thaw the reagents completely. Mix 0.2 µL of RT Enzyme with 14.8 µL of the IMNV SuperMix and pipette 15 µL to each well of the PCR tube strip or well plate.
Mix 5 µL of the purified RNA sample and you are ready to run the PCR reaction.
When ready to use, thaw the reagents completely. Mix 0.2 µL of RT Enzyme with 14.8 µL of the IMNV SuperMix and pipette 15 µL to each well of the PCR tube strip or well plate.
Mix 5 µL of the purified RNA sample and you are ready to run the PCR reaction.
Resources
PDF (3.74 MB)
PDF (0.22 MB)
FAQ
Which best organs or tissue that are recommended for use in IMNV PCR test?
IMNV infects tissues of mesodermal origin. The principal target tissues in the acute phase of infection with IMNV are the striated muscles (skeletal and less commonly cardiac muscle), connective tissues, haemocytes, and the lymphoid organ tubule parenchymal cells. In chronic infections, the lymphoid organ may be the principal target tissue.
What are the extraction methods that can be used to perform the qPCR test?
This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction. Achieve optimal performance by pairing this qPCR Kit with our ShrimProtect X-Kit.
What qPCR channel is being used in the testing?
FAM/Green: ORF1 (IMNV)
Cy5/Red: Shrimp Internal Control
Cy5/Red: Shrimp Internal Control
How many times can the reagent be freeze-thawed during its shelf-life?
Up to 8 times during the product's shelf-life.
Can the mixture of RT enzyme with SuperMix be stored after mixing?
No, mixing RT enzyme with SuperMix must be freshly prepared before qPCR testing.
Nisa
We at Sumber Hatchery Bangka have been using Nusantics' ShrimProtect test-kit for almost 1.5 years to test larvae, feed, and worms, and we’ve experienced many advantages compared to other test-kits. First, the reagents are easy to use; second, it is more sensitive; and third, the PCR results are more accurate.
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