PathoScan hrHPV

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PathoScan hrHPV

PathoScan hrHPV

PathoScan hrHPV qPCR Kit utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect 14 high-risk HPV and genotype HPV52, HPV16, HPV18 from cervical swab and urine samples in under 35 minutes.

Clinical Performance

Sensitivity: 96.55% (cervical swabs), 80.88% (urine)
Specificity: 99.87% (cervical swabs), 100% (urine)
Positive Predictive Value: 98.25% (cervical swabs), 100% (urine)
Negative Predictive Value: 99.75% (cervical swabs), 98.38% (urine)

Sample Type

Purified DNA from cervical swabs or urine

Instrument Compatibility

QuantStudio 5
BioRad CFX-96/CFX Opus
RotorGene Q
Tianlong Gentier 96E
PCR system with FAM, HEX, Texas Red-X, Cy5, and Cy5.5 channels

Features

Detects E6 and E7 gene target: Can detect 14 types of high-risk HPV in all infection stages
ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
Open System: Use any existing qPCR system that you have
Speedy: Only 33 minutes PCR time (with QuantStudio 5)

Product Details

PathoScan hrHPV qPCR Kit has acquired certification from KEMENKES RI AKD 20306320321 to detect 14 high-risk HPV that cause cervical cancer. PathoScan hrHPV can identify HPV 16, HPV 18, HPV 52, and HPV Others (HPV 31, 33, 35, 39, 45, 51, 56, 58, 59, 66, 68) with β-globin as the Internal Control. The Limit of Detection (LoD) ranges from 0.5 to 1.25 copies/µL reaction with a clinical accuracy of 99.65% for cervical swabs and 98.48% for urine samples.

Procedures

Store the reagents at -20^oC upon delivery.

When ready to use, thaw completely and pipette 15 µL to each well of the PCR tube strip or well plate.

Mix 5 µL of the purified DNA and you are ready to run the PCR reaction.

Resources

Product Sheet PathoScan hrHPV qPCR Kit (ENG)

PDF (5.01 MB)

Brochure PathoScan hrHPV qPCR Kit (ENG)

PDF (3.12 MB)

Product Sheet PathoScan hrHPV qPCR Kit (IND)

PDF (5.29 MB)

Brochure PathoScan hrHPV qPCR Kit (IND)

PDF (3.36 MB)

FAQ

Which organs can be infected by HPV?

HPV infection affects epithelial cells and can lead to dysplasia of the cervix, anus, oropharynx, penis, vagina, and vulva.

What are the extraction methods that can be used to perform the qPCR test?

This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction.

What qPCR channel is being used in the testing?

Cy5.5/Far Red: β-globin (Internal Control)
HEX/Yellow: HPV16
TEX/Orange: HPV18
Cy5/Red: HPV52
FAM/Green: HPV31, 33, 35,39,45,51,56,58,59,66,68

Why can urine be used as an HPV DNA specimen?

Urine that can be used as an HPV DNA test specimen is the first void urine/first stream urine. First void urine is preferred as it contains a higher concentration of HPV DNA compared to random or midstream urine due to its ability to collect most of the cellular debris from the urogenital tract.

Can I use PCR that does not have the Cy5.5 channel in testing using the PathoScan hrHPV qPCR Kit?

No, because Cy5.5 is used to detect β-globin as an internal control. If the PCR instrument does not have a Cy5.5 channel, a crosstalk may potentially occur with the Cy5 channel.

What sets PathoScan hrHPV qPCR test apart from other tests like the Pap Smear? Is the obtained data more accurate?

PathoScan hrHPV qPCR relies on HPV DNA detection, whereas the Pap smear is based on the observation of cervical cells. Due to its focus on HPV DNA, PathoScan hrHPV qPCR Kit enhances the likelihood of early detection of cervical cancer.

Ready to Order?

Take a look at what our customers mention about our Products & Solutions

dr. Ricky Tjahjadi, Sp.PK

Early detection of HPV is crucial, as this virus is responsible for 99% of cervical cancer cases. Thanks to Nusantics' innovation, the test can now be performed using just a urine sample, enabling us to detect and prevent cancer before it develops, which significantly increases the chances of patient recovery