ShrimProtect TSV

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ShrimProtect TSV

ShrimProtect TSV

ShrimProtect RxReady TSV qRT-PCR Kit utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect Taura Syndrome Virus (TSV) in various samples in under 45 minutes.

Analytical Performance

Limit of Detection (LoD): 0.5 copies/µL reaction.
Good linearity (R² > 0.99) from 100,000 copies/reaction until 10 copies/reaction.

Sample Type

Purified RNA from broodstock, shrimp fries, shrimps, artemia, polychaeta, water, mud, and feed.

Instrument Compatibility

QuantStudio 5
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red, and Cy5 channels

Features

ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
Open System: Use any existing qPCR system that you have
Speedy: Only 43 minutes PCR time (with QuantStudio 5)

Product Details

ShrimProtect RxReady TSV qRT-PCR Kit has been validated by Balai Pengujian Ikan & Lingkungan (BPKIL) and has acquired certificates NIE KKP RI D 2309755 BBC to specifically detect Capsid gene of TSV. The kit also contains Internal Control for shrimp.

Procedures

Store the reagents at -20^oC upon delivery.

When ready to use, thaw the reagents completely. Mix 0.2 µL of RT Enzyme with 14.8 µL of the TSV SuperMix and pipette 15 µL to each well of the PCR tube strip or well plate.

Mix 5 µL of the purified RNA and you are ready to run the PCR reaction.

Resources

Brochure ShrimProtect

PDF (0.22 MB)

FAQ

Which organs or tissue that are recommended for use in TSV PCR test?

Suitable specimens for testing for infection with TSV include post-larvae, juveniles and adults. TSV infects tissues of ectodermal and mesodermal origin. The principal target tissue in the acute phase of infection with TSV is the cuticular epithelium.

What are the extraction methods that can be used to perform the qPCR test?

This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction. Achieve optimal performance by pairing this qPCR Kit with our ShrimProtect X-Kit.

What qPCR channel is being used in the testing?

FAM/Green: Capsid (TSV)
Cy5/Red: Shrimp Internal Control

How many times can the reagent be freeze-thawed during its shelf-life?

Up to 8 times during the product's shelf-life.

Can the mixture of RT enzyme with SuperMix be stored after mixing?

No, mixing RT enzyme with SuperMix must be freshly prepared before qPCR testing.

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