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ShrimProtect AHPND/EMS

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ShrimProtect AHPND/EMS

ShrimProtect RxReady AHPND qPCR Kit utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect target genes pirA and pirB in various samples in under 35 minutes.

Analytical Performance

Limit of Detection (LoD): 0.5-0.75 copies/uL reaction.
Good linearity (R2 > 0.99) from 100,000 copies/reaction to 100 copies/reaction.

Sample Type

Purified DNA from broodstock, shrimp fries, shrimps, artemia, polychaeta, water, mud, and feed.

Instrument Compatibility

QuantStudio 5
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red-X, and Cy5 channels

Features

  • ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
  • Open System: Use any existing qPCR system that is available in your laboratory
  • Speedy: Only 33 minutes PCR time (with QuantStudio 5).

Product Details

ShrimProtect RxReady AHPND qPCR Kit has been validated by Balai Pengujian Ikan & Lingkungan (BPKIL) and has acquired certificates NIE KKP RI D 2212684 BBC to specifically detect pirA and pirB genes of AHPND/EMS-causing Vibrio parahaemolyticus (VPAHPND). The kit also contains Internal Control for shrimp.

Procedures

Store the reagents at -20oC upon delivery.

When ready to use, thaw completely and pipette 15 µL to each well of the PCR tube strip or well plate.

Mix 5 µL of the purified DNA and you are ready to run the PCR reaction.
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Resources

FAQ

This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction. Achieve optimal performance by pairing this qPCR Kit with our ShrimProtect X-Kit.
FAM/Green: pirA
HEX/Yellow: pirB
Cy5/Red: Shrimp Internal Control
Up to 8 times during the product's shelf-life.
The pirA protein play an auxiliary role in the pathogenesis of AHPND, while pirB alone could cause cellular damage, such as necrosis, apoptosis and other pathophysiological damage associated with AHPND.
Samples are positive if there is amplification of pirA and/or pirB genes and it is positive as long as the Ct value is under 40.

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ShrimProtect AHPND/EMS
ShrimProtect AHPND/EMS

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