ShrimProtect AHPND/EMS
ShrimProtect RxReady AHPND qPCR Kit utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect target genes pirA and pirB in various samples in under 35 minutes.
Analytical Performance
Limit of Detection (LoD): 0.5-0.75 copies/uL reaction.
Good linearity (R2 > 0.99) from 100,000 copies/reaction to 100 copies/reaction.
Good linearity (R2 > 0.99) from 100,000 copies/reaction to 100 copies/reaction.
Sample Type
Purified DNA from broodstock, shrimp fries, shrimps, artemia, polychaeta, water, mud, and feed.
Instrument Compatibility
QuantStudio 5
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red-X, and Cy5 channels
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red-X, and Cy5 channels
Features
- ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
- Open System: Use any existing qPCR system that is available in your laboratory
- Speedy: Only 33 minutes PCR time (with QuantStudio 5).
Product Details
ShrimProtect RxReady AHPND qPCR Kit has been validated by Balai Pengujian Ikan & Lingkungan (BPKIL) and has acquired certificates NIE KKP RI D 2212684 BBC to specifically detect pirA and pirB genes of AHPND/EMS-causing Vibrio parahaemolyticus (VPAHPND). The kit also contains Internal Control for shrimp.
Procedures
Store the reagents at -20oC upon delivery.
When ready to use, thaw completely and pipette 15 µL to each well of the PCR tube strip or well plate.
Mix 5 µL of the purified DNA and you are ready to run the PCR reaction.
When ready to use, thaw completely and pipette 15 µL to each well of the PCR tube strip or well plate.
Mix 5 µL of the purified DNA and you are ready to run the PCR reaction.
Resources
PDF (4.32 MB)
PDF (0.22 MB)
FAQ
Which organs or tissue that are recommended for use in AHPND PCR test?
Samples of gut-associated tissues and organs, such as hepatopancreas, stomach, the midgut and the hindgut are suitable for testing. In addition, faecal (non-lethal) samples may be collected from valuable broodstock.
What are the extraction methods that can be used to perform the qPCR test?
This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction. Achieve optimal performance by pairing this qPCR Kit with our ShrimProtect X-Kit.
What qPCR channel is being used in the testing?
FAM/Green: pirA
HEX/Yellow: pirB
Cy5/Red: Shrimp Internal Control
HEX/Yellow: pirB
Cy5/Red: Shrimp Internal Control
How many times can the reagent be freeze-thawed during its shelf-life?
Up to 8 times during the product's shelf-life.
What is the difference between pirA and pirB and the correlation with the occurrence of AHPND in shrimp?
The pirA protein play an auxiliary role in the pathogenesis of AHPND, while pirB alone could cause cellular damage, such as necrosis, apoptosis and other pathophysiological damage associated with AHPND.
How to interpret AHPND positive in the samples?
Samples are positive if there is amplification of pirA and/or pirB genes and it is positive as long as the Ct value is under 40.
Nisa
We at Sumber Hatchery Bangka have been using Nusantics' ShrimProtect test-kit for almost 1.5 years to test larvae, feed, and worms, and we’ve experienced many advantages compared to other test-kits. First, the reagents are easy to use; second, it is more sensitive; and third, the PCR results are more accurate.
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