
VarScreen RxReady
VarScreen RxReady is designed to detect and differentiate SARS-CoV-2 variants through an innovative combination of gene detection nsp-13 (helicase), 4 SGTF (S-Gene Target Failure) targets and 1 ORF1ab 3675 deletion.
Clinical Performance
Sensitivity: ≥ 95%*
Specificity: ≥ 95%*
Positive Predictive Value: ≥ 95%*
Negative Predictive Value: ≥ 95%*
*up to 100% in samples with Ct < 30 compared with Whole Genome Sequencing (WGS)
Specificity: ≥ 95%*
Positive Predictive Value: ≥ 95%*
Negative Predictive Value: ≥ 95%*
*up to 100% in samples with Ct < 30 compared with Whole Genome Sequencing (WGS)
Sample Type
Purified RNA from NP, OP, NPOP swabs, and gargle samples
Instrument Compatibility
QuantStudio 5
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red, Cy5 channels.
BioRad CFX-96/CFX Opus
PCR system with FAM, HEX, Texas Red, Cy5 channels.
Features
- Speedy: Under 55 minutes with QuantStudio 5.
- Variant Surveillance : Monitoring Variants-of-Concern and Variant-of-Interest for effective infection control
- Multiplex Real Time PCR : Accurate multiplex test for detection and variant identification of SARS-CoV-2 with 4 S gene mutations and 1 ORF1ab deletion
- Highly Flexible : Ready to use in any open PCR system (with 4 channels detection)
Product Details
VarScreen RxReady has acquired certification from Ministry of Health (KEMENKES RI AKD 20303220035) to accurately detect and genotype the SARS-CoV-2 variant by detecting the SNPs polymorphism and deletion through the SGTF method (S-gene Target Failure). VarScreen RxReady has 4 SGTFs (H69/V70 deletion, T478K, E484A/K/Q, N501Y) and 1 ORF1ab deletion (SGF3675-3677) detection. This kit has a Limit of Detection (LoD) of 0.5 copies/µL reaction.
Procedures
Store the reagents at -20oC upon delivery.
When ready to use, thaw the reagents completely. Prepare VarScreen Reaction 1 by mixing 0.2 µL of RT Enzyme with 14.8 µL of the VarScreen Reaction 1. Repeat the steps before with VarScreen Reaction 2. Mix well and pipette 15 µL of VarScreen Reaction 1 and Reaction 2 to each well of the PCR tube strip or well plate.
Each sample must be tested with VarScreen Reaction 1 and VarScreen Reaction 2. Mix 5 µL of the purified RNA sample and you are ready to run the PCR reaction.
When ready to use, thaw the reagents completely. Prepare VarScreen Reaction 1 by mixing 0.2 µL of RT Enzyme with 14.8 µL of the VarScreen Reaction 1. Repeat the steps before with VarScreen Reaction 2. Mix well and pipette 15 µL of VarScreen Reaction 1 and Reaction 2 to each well of the PCR tube strip or well plate.
Each sample must be tested with VarScreen Reaction 1 and VarScreen Reaction 2. Mix 5 µL of the purified RNA sample and you are ready to run the PCR reaction.

FAQ
What are the extraction methods that can be used to perform the qPCR test?
This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction. Achieve optimal performance by pairing this qPCR Kit with our Nusantics X-Kit.
What qPCR channel is being used in the testing?
FAM/Green: Helicase & E484K/Q/A
HEX/Yellow: N501Y
TEX/Orange: RPP30 & T478K
Cy5/Red: H69/V70- & ORI1abSGF3675-3677
HEX/Yellow: N501Y
TEX/Orange: RPP30 & T478K
Cy5/Red: H69/V70- & ORI1abSGF3675-3677
How many times can the reagent be freeze-thawed during its shelf-life?
Up to 8 times during the product's shelf-life.
What SARS-CoV-2 variants can VarScreen RxReady detect?
VarScreen RxReady is capable of detecting and characterizing 4 mutations in the S gene and 1 deletion in the ORF1ab region of SARS-CoV-2 such as Alpha (B.1.1.7), Beta (B.1.351), Indo Variant (B.1466.2), Delta (B.1617.2), Omicron (BA.1/BA.2), and Eris (EG.5).
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