PathoScan hrHPV Lite

Home

Products & Solutions

Human Diagnostics

Reproductive Health

PathoScan hrHPV Lite

PathoScan hrHPV qPCR Kit Lite utilizes our advanced proprietary Fenrir Mastermix with specific fluorescence probes to accurately detect 14 types of high-risk HPV and genotype 2 high-risk HPV (HPV16 and 18) from cervical swab and urine samples in under 35 minutes.

Clinical Performance

Sensitivity: 96.55% (cervical swabs), 80.88% (urine)
Specificity: 99.87% (cervical swabs), 100% (urine)
Positive Predictive Value: 98.25% (cervical swabs), 100% (urine)
Negative Predictive Value: 99.75% (cervical swabs), 98.38% (urine)

Sample Type

Purified DNA from cervical swabs or urine

Instrument Compatibility

QuantStudio 5
BioRad CFX-96/CFX Opus
Tianlong Gentier 48E
LightCycler® 96 Roche
Rotor Gene Q
PCR system with FAM, HEX, Texas Red, and Cy5 channels

Features

Detects E6 and E7 gene target: Can detect 14 types of high-risk HPV in all infection stages
ReadyMix format: No more mixing in the lab! Just thaw the reagents and pipette them into PCR tube strips or well plates
Open System: Use any existing qPCR system that you have
Speedy: Only 33 minutes PCR time (with QuantStudio 5)

Product Details

PathoScan hrHPV qPCR Kit Lite has acquired certification from KEMENKES RI AKD 20306420161 to detect 14 high-risk HPV that cause cervical cancer. PathoScan hrHPV qPCR Kit Lite can identify HPV16, HPV18 and HPV Others (HPV31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) with β-globin as the Internal Control. The Limit of Detection (LoD) ranges from 0.5 to 1.25 copies/µL reaction with a clinical accuracy of 99.65% for cervical swabs and 98.48% for urine samples.

Procedures

Store the reagents at -20^oC upon delivery.

When ready to use, thaw completely and pipette 15 µL to each well of the PCR tube strip or well plate.

Mix 5 µL of the purified DNA and you are ready to run the PCR reaction.

FAQ

Which organs can be infected by HPV?

HPV infection affects epithelial cells and can lead to dysplasia of the cervix, anus, oropharynx, penis, vagina, and vulva.

What are the extraction methods that can be used to perform the qPCR test?

This qPCR Kit is compatible with various extraction methods, including commercially available spin column extraction.

What qPCR channel is being used in the testing?

Cy5/Red : β-globin (Internal Control)
HEX/ Yellow: HPV16
TEX/ Orange: HPV18
FAM/Green : HPV31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68

Why can urine be used as an HPV DNA specimen?

Urine that can be used as an HPV DNA test specimen is the first void urine/first stream urine. First void urine is preferred as it contains a higher concentration of HPV DNA compared to random or midstream urine due to its ability to collect most of the cellular debris from the urogenital tract.

What sets PathoScan hrHPV qPCR Kit Lite test apart from other tests like the Pap Smear? Is the obtained data more accurate?

PathoScan hrHPV qPCR Kit Lite relies on HPV DNA detection, whereas the Pap smear is based on the observation of cervical cells. Due to its focus on HPV DNA, PathoScan hrHPV qPCR Kit enhances the likelihood of early detection of cervical cancer.

What is the difference between the PathoScan hrHPV qPCR Kit Lite and the PathoScan hrHPV qPCR Kit?

The PathoScan hrHPV qPCR Kit Lite can be tested using 4 qPCR channels: HEX, TEX, FAM, and Cy5. In addition, it can perform genotyping for HPV 16, HPV 18, and others (HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). Meanwhile, the PathoScan hrHPV qPCR Kit shall be tested using qPCR channels: HEX, TEX, FAM, Cy5, and Cy5.5. In addition, it can perform genotyping for HPV 52, HPV 16, HPV 18, and others (HPV 31, 33, 35, 39, 45, 51, 56, 58, 59, 66, and 68).

Ready to Order?

Get the best offer for your diagnosis needs!