ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

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ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

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ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

ShrimProtect X-Kit V2.0 utilizes silica-based spin column extraction method to produce higher yield of total nucleic acid (DNA & RNA) for an increased pathogen detection sensitivity, while maintaining its purification quality.

Kit Components

Preparation Buffer (60 mL/bottle)
Spin Column (50 pieces/pack)
Collection Tube (100 pieces/pack)
Lysis Buffer (24 mL/bottle)
Wash Buffer 1 (concentrate) (27 mL/bottle)
Wash Buffer 2 (concentrate) (18 mL/bottle)
Elution Buffer (12 mL/bottle)
Proteinase K (2.4 mL/bottle)
Carrier RNA (350 μL/tube)
Spiked-In IC (480 μL/tube)

Features

Simple-to-Use: Only requires 5 stages from sample preparation to obtain purified DNA/RNA samples
Speedy: Sample processing only takes 30-40 minutes (for 9 samples)
Extract total nucleic acid (DNA and RNA) in high yield and purity

Product Details

The ShrimProtect X-Kit utilizes a silica-based spin column to extract and enrich total nucleic acids (DNA and RNA) with high yield and purity from a wide range of samples, including shrimp and water. The silica solid phase binds nucleic acids under specific conditions, allowing for the washing and elution of DNA and RNA with high purity, free from contaminants.

Procedures

Kit storage condition: Store at room temperature (20 - 25°C). Proteinase K needs to be stored between 2-8°C and Carrier RNA needs to be stored between -25°C to -15°C.

Sample Preparation:
- Solid Samples: Add the samples to be extracted into a 1.5 mL tube. Use a sterile tissue grinder to grind the samples until a fine consistency is achieved.
- Liquid Samples: Add liquid samples into a sterile 1.5 mL microtube, then centrifuge it to precipitate liquid samples.
Preparation Buffer: Prepare the samples by adding the Preparation Buffer according to the instructions in the manual.
Lysis Steps:
- Transfer the prepared samples to the Lysis Buffer Mix, which includes Lysis Buffer, Carrier RNA, and Proteinase K.
- Incubate the samples for lysis at 56°C for 5 minutes.
Extraction Protocol: Follow the extraction protocol as detailed in the manual.
Elution:
- Elute the purified total nucleic acid with 50-100 µL of Eluent Buffer.
- Store the nucleic acid elution at ≤ -20°C.

This streamlined process ensures the efficient extraction and preservation of nucleic acids from your samples.

FAQ

Absolute ethyl alcohol.

Sterile centrifuge tube of 1.5 mL (microtube), sterile centrifuge tube of 15 mL (falcon), and sterile scalpel blade.

Sterile tissue grinder, centrifuge, vortex, spindown, and sterile tweezer.

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ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

ShrimProtect X-Kit V2.0: Total DNA/RNA Extraction Kit

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Sample Type

Gill, pleopod, shrimp head, whole shrimp, artemia, oyster, squid, blood worm, polychaeta, feed, water, and mud