ShrimProtect X-Kit v1.0

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ShrimProtect X-Kit v1.0

ShrimProtect X-Kit utilizes Silica Column extraction method to produce total nucleic acid (DNA & RNA) with high purity and high concentration for an increased pathogen detection sensitivity.

Kit Components

Preparation Buffer (1 x 60 mL)
Spin Column (2 x 48 tubes)
Collection Tube (4 x 96 tubes)
Lysis Buffer (2 x 11 mL)
IR Buffer (2 x 16.5 mL)
Wash Buffer (2 x 11 mL)
Eluent Buffer (2 x 6 mL)
Proteinase K (2 x 2.7 mL)
Lyophilized Carrier RNA (2 x 155 µg)

Sample Type

Gill, pleopod, shrimp head, whole shrimp, artemia, oyster, polychaeta, squid, crab, water, and mud

Features

Simple-to-Use: only requires 5 stages from sample preparation to obtaining purified RNA/DNA samples.
Speedy: Sample processing only takes 30-40 minutes (for 9 samples).
Extract total nucleic acid (DNA and RNA) in high yield and high purity.

Product Details

The ShrimProtect X-Kit utilizes a silica-based spin column to extract and enrich total nucleic acids (DNA and RNA) with high yield and purity from a wide range of samples, including shrimp and water. The silica solid phase binds nucleic acids under specific conditions, allowing for the washing and elution of DNA and RNA with high purity, free from contaminants.

Procedures

Kit storage condition: Store at room temperature (20 - 25^oC). Carrier RNA should be stored at -20^oC±5^oC after dissolution.

Sample Grinding: Add the samples to be extracted into a 1.5 mL tube. Use tissue grinder consumables to grind the samples until a fine consistency is achieved.
Preparation Buffer: Prepare the samples by adding the Preparation Buffer according to the instructions in the manual.
Lysis Step:
- Transfer the prepared samples to the Lysis Buffer Mix, which includes Lysis Buffer, carrier RNA, and Proteinase K.
- Incubate the samples for lysis at 72°C for 5 minutes.
Extraction Protocol: Follow the extraction protocol as detailed in the manual.
Elution:
- Elute the purified total nucleic acid with 50 µL of Eluent Buffer.
- Store the eluted nucleic acid at -20°C.

This streamlined process ensures the efficient extraction and preservation of nucleic acids from your samples.